To our knowledge, this is the first report providing evidence to support the use of an RPA-LFD assay as a specific and sensitive platform for detecting CAEV proviral DNA in goats in a faster manner, one that is also applicable for on-site utilization at farms and that should be useful in both eradication programs and epidemiological studies. When the RPA-LFD was applied in parallel with serological ELISA for the detection of CAEV in field samples, the RPA-LFD assay exhibited a higher sensitivity than the traditional method, and 82% of the 200 samples collected in Taiwan were found to be positive. Given its simplicity and portability, this RPA-LFD protocol can serve as an alternative tool to ELISA for the primary screening of CAEV, one that is suitable for both laboratory and field application. Furthermore, there was no cross-reaction with other tested viruses, including goat pox virus and bovine leukemia virus. Under the optimal incubation conditions, specifically, 30min at 37Â☌ for RPA followed by 5min at room temperature for LFD, the assay was found to be sensitive to a lower limit of 80pg of total DNA and 10 copies of plasmid DNA. Hence, a more efficient detection platform based on recombinase polymerase amplification (RPA) and a lateral flow dipstick (LFD) was developed in the present study for detecting the proviral DNA of caprine arthritis-encephalitis virus (CAEV). The prognosis for the encephalitic form is normally poor, and this form of the disease has caused substantial economic losses for goat farmers.

This persistent viral infection results in arthritis in adult goats and encephalitis in lambs. Tu, Po-An Shiu, Jia-Shian Lee, Shu-Hwae Pang, Victor Fei Wang, De-Chi Wang, Pei-HwaĬaprine arthritis-encephalitis ( CAE) in goats is a complex disease syndrome caused by a lentivirus. Images Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 1 Figure 2 Figure 3 Figure 4 PMID:7406019ĭevelopment of a recombinase polymerase amplification lateral flow dipstick (RPA-LFD) for the field diagnosis of caprine arthritis-encephalitis virus (CAEV) infection. Microbiologic techniques failed to demonstrate any bacteria, mycoplasma, or chlamydia in the lesions. Amyloid was also found in liver, spleen, and kidney. The inflammatory infiltrates in these organs were predominantly mononuclear. Extrasynovial lesions were located in kidneys, vessels, and brain. Lesions were most often associated with synovial-lined structures including joints, tendon sheaths, and bursae, and were typified by synovial cell proliferations, subsynovial mononuclear cell infiltration, the presence of fibrin, fibrinous concretions, necrosis, and mineralization. The gross and microscopic connective tissue lesions in 12 goats with caprine arthritis-encephalitis ( CAE) are described, including those from which a virus (CAEV) was isolated.

The connective tissue component of the caprine arthritis-encephalitis syndrome.Ĭrawford, T.

Caprine cross 2.0